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cell titer glow reagent molecular cell 85, 1–13.e1–e11, july 3, 2025 e8  (Promega)

 
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    Structured Review

    Promega cell titer glow reagent molecular cell 85, 1–13.e1–e11, july 3, 2025 e8
    Cell Titer Glow Reagent Molecular Cell 85, 1–13.E1–E11, July 3, 2025 E8, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+titer+glow/cell+titer+glow+reagent+molecular+cell+85++1+13+e1+e11++july+3++2025+e8/pm40532701-391-20-22
    Average 90 stars, based on 1 article reviews
    cell titer glow reagent molecular cell 85, 1–13.e1–e11, july 3, 2025 e8 - by Bioz Stars, 2026-10
    90/100 stars

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    other:

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    Article Snippet: NF-κB activity was determined by assessing the levels of Secreted Alkaline Phosphatase (SEAP) in the supernatant, as measured by the Quanti-Blue assay (Invivogen, San Diego, CA USA) and performed according to manufacturer's instructions.

    Cytotoxicity Assay:

    Article Title: Targeting Parallel Topology of G-Quadruplex Structures by Indole- Fused Quindoline Scaffolds
    Article Snippet: All the media, serum, antibiotics and reagents used for cell-based experiments were purchased from HiMedia, MP Biomedicals and Glibco. .. The reagent used for cytotoxicity assay, Cell titer Glow was purchased from Promega. ..

    Magnetic Beads:

    Article Title: Structural basis for the midnolin-proteasome pathway and its role in suppressing myeloma.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Actin (RRID: AB_2223172) Cell Signaling Technology 4970 Rabbit anti-PSMD2 (RRID: AB_2798903) Cell Signaling Technology 25430 Rabbit anti-PSMA2 (RRID: AB_2171400) Cell Signaling Technology 2455 Rabbit anti-PSMB5 (RRID: AB_2798061) Cell Signaling Technology 12919 Rabbit anti-HA (RRID: AB_1549585) Cell Signaling Technology 3724 Rabbit monoclonal anti-FLAG (RRID: AB_2572291) Cell Signaling Technology 14793 Mouse monoclonal anti-FLAG (RRID: AB_262044) Sigma Aldrich F1804 Rabbit anti-mTOR (RRID: AB_2105622) Cell Signaling Technology 2983 Rabbit anti-TNPO1 (RRID: AB_3662993) Cell Signaling Technology 31452 Rabbit anti-KPNA2 (RRID: AB_2798467) Cell Signaling Technology 14372 Rabbit anti-IRF4 (RRID: AB_10547141) Cell Signaling Technology 4299 anti-rabbit IgG, HRP-linked (RRID: AB_2099233) Cell Signaling Technology 7074 anti-mouse Alexa 488-conjugated (RRID: AB_2534069) Thermo Fisher Scientific A-11001 anti-FLAG (RRID: AB_2637089) magnetic beads Sigma M8823 anti-HA (RRID: AB_2749815) magnetic beads Thermo Fisher Scientific 88836 M2 FLAG agarose resin Millipore A2220 NeutrAvidin Agarose Thermo Fischer Scientific 29200 Ni-NTA agarose Qiagen 30210 IRDye 800CW Streptavidin LI-COR 926-32230 Bacterial and viral strains Rosetta (DE3) cells Millipore 71397-3 Chemicals, peptides, and recombinant proteins MG132 Selleckchem S2619 PMA Thermo Fisher Scientific J63916.MA Hoechst 33342 Thermo Fisher Scientific H3570 IPTG Sigma I5502-10G Imidazole Sigma 68268-500ML-F Dithiothreitol Thermo Fisher Scientific R0862 Benzonase Millipore 71206-3 HisTEV Thermo Fisher Scientific 12-575-015 Coomassie Brilliant Blue R-250 staining solution Bio Rad 1610436 Tris-Glycine SDS sample buffer Thermo Fisher Scientific LC2676 Immunofluorescence blocking buffer LI-COR 927-70001 ProLong Glass antifade mountant Thermo Fisher Scientific P36982 Protease inhibitor tablets Sigma 11873580001 HALT protease/phosphatase inhibitor Thermo Fisher Scientific 78441 Pierce ECL western blotting substrate Thermo Fisher Scientific 32106 3xFLAG peptide APExBIO A6001 Sequencing Grade Trypsin Promega V5113 Critical commercial assays Cell Titer Glow Promega G9242 PolyJet Transfection Reagent SignaGen SL100688 Q5 Site Directed Mutagenesis kit New England Biolabs E0554S (Continued on next page) e1 Molecular Cell 85, 1–13.e1–e11, July 3, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Gibson Assembly Master Mix New England Biolabs E2611S RNeasy Plus Mini Kit Qiagen 74134 iScript cDNA Synthesis Kit Bio Rad 1708891 Platinum SYBR Green Thermo Fisher Scientific 11733038 S-Trap Micro Proteomics Sample Prep Columns Protifi, LLC C02-micro-10 Deposited data Midnolin-proteasome M1A cryo-EM model and map This paper PDB: 9BUI; EMDB ID: EMD-44910 Midnolin-proteasome M1B cryo-EM model and map This paper PDB: 9BV3; EMDB ID: EMD-44930 Midnolin-proteasome M2A cryo-EM model and map This paper PDB: 9BV1; EMDB ID: EMD-44927 Midnolin-proteasome M2A (focused) cryo-EM map This paper EMDB ID: EMDB ID: EMD-44928 Midnolin-proteasome M2B cryo-EM model and map This paper PDB: 9BV2; EMDB ID: EMD-44929 EGR1-Catch domain crystal structure This paper PDB: 8VQP Raw images and mass spectrometry files This paper Mendeley Data: https://doi.org/10.17632/ xn3xtj2xb7.1 Experimental models: Cell lines HEK-293T ATCC CRL-3216 AMO-1 DSMZ ACC 538 JJN3 DSMZ ACC 541 KMS27 JCRB JCRB1188 KMS26 JCRB JCRB1187 ANBL6 Sigma SCC429

    Recombinant:

    Article Title: Structural basis for the midnolin-proteasome pathway and its role in suppressing myeloma.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Actin (RRID: AB_2223172) Cell Signaling Technology 4970 Rabbit anti-PSMD2 (RRID: AB_2798903) Cell Signaling Technology 25430 Rabbit anti-PSMA2 (RRID: AB_2171400) Cell Signaling Technology 2455 Rabbit anti-PSMB5 (RRID: AB_2798061) Cell Signaling Technology 12919 Rabbit anti-HA (RRID: AB_1549585) Cell Signaling Technology 3724 Rabbit monoclonal anti-FLAG (RRID: AB_2572291) Cell Signaling Technology 14793 Mouse monoclonal anti-FLAG (RRID: AB_262044) Sigma Aldrich F1804 Rabbit anti-mTOR (RRID: AB_2105622) Cell Signaling Technology 2983 Rabbit anti-TNPO1 (RRID: AB_3662993) Cell Signaling Technology 31452 Rabbit anti-KPNA2 (RRID: AB_2798467) Cell Signaling Technology 14372 Rabbit anti-IRF4 (RRID: AB_10547141) Cell Signaling Technology 4299 anti-rabbit IgG, HRP-linked (RRID: AB_2099233) Cell Signaling Technology 7074 anti-mouse Alexa 488-conjugated (RRID: AB_2534069) Thermo Fisher Scientific A-11001 anti-FLAG (RRID: AB_2637089) magnetic beads Sigma M8823 anti-HA (RRID: AB_2749815) magnetic beads Thermo Fisher Scientific 88836 M2 FLAG agarose resin Millipore A2220 NeutrAvidin Agarose Thermo Fischer Scientific 29200 Ni-NTA agarose Qiagen 30210 IRDye 800CW Streptavidin LI-COR 926-32230 Bacterial and viral strains Rosetta (DE3) cells Millipore 71397-3 Chemicals, peptides, and recombinant proteins MG132 Selleckchem S2619 PMA Thermo Fisher Scientific J63916.MA Hoechst 33342 Thermo Fisher Scientific H3570 IPTG Sigma I5502-10G Imidazole Sigma 68268-500ML-F Dithiothreitol Thermo Fisher Scientific R0862 Benzonase Millipore 71206-3 HisTEV Thermo Fisher Scientific 12-575-015 Coomassie Brilliant Blue R-250 staining solution Bio Rad 1610436 Tris-Glycine SDS sample buffer Thermo Fisher Scientific LC2676 Immunofluorescence blocking buffer LI-COR 927-70001 ProLong Glass antifade mountant Thermo Fisher Scientific P36982 Protease inhibitor tablets Sigma 11873580001 HALT protease/phosphatase inhibitor Thermo Fisher Scientific 78441 Pierce ECL western blotting substrate Thermo Fisher Scientific 32106 3xFLAG peptide APExBIO A6001 Sequencing Grade Trypsin Promega V5113 Critical commercial assays Cell Titer Glow Promega G9242 PolyJet Transfection Reagent SignaGen SL100688 Q5 Site Directed Mutagenesis kit New England Biolabs E0554S (Continued on next page) e1 Molecular Cell 85, 1–13.e1–e11, July 3, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Gibson Assembly Master Mix New England Biolabs E2611S RNeasy Plus Mini Kit Qiagen 74134 iScript cDNA Synthesis Kit Bio Rad 1708891 Platinum SYBR Green Thermo Fisher Scientific 11733038 S-Trap Micro Proteomics Sample Prep Columns Protifi, LLC C02-micro-10 Deposited data Midnolin-proteasome M1A cryo-EM model and map This paper PDB: 9BUI; EMDB ID: EMD-44910 Midnolin-proteasome M1B cryo-EM model and map This paper PDB: 9BV3; EMDB ID: EMD-44930 Midnolin-proteasome M2A cryo-EM model and map This paper PDB: 9BV1; EMDB ID: EMD-44927 Midnolin-proteasome M2A (focused) cryo-EM map This paper EMDB ID: EMDB ID: EMD-44928 Midnolin-proteasome M2B cryo-EM model and map This paper PDB: 9BV2; EMDB ID: EMD-44929 EGR1-Catch domain crystal structure This paper PDB: 8VQP Raw images and mass spectrometry files This paper Mendeley Data: https://doi.org/10.17632/ xn3xtj2xb7.1 Experimental models: Cell lines HEK-293T ATCC CRL-3216 AMO-1 DSMZ ACC 538 JJN3 DSMZ ACC 541 KMS27 JCRB JCRB1188 KMS26 JCRB JCRB1187 ANBL6 Sigma SCC429

    Staining:

    Article Title: Structural basis for the midnolin-proteasome pathway and its role in suppressing myeloma.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Actin (RRID: AB_2223172) Cell Signaling Technology 4970 Rabbit anti-PSMD2 (RRID: AB_2798903) Cell Signaling Technology 25430 Rabbit anti-PSMA2 (RRID: AB_2171400) Cell Signaling Technology 2455 Rabbit anti-PSMB5 (RRID: AB_2798061) Cell Signaling Technology 12919 Rabbit anti-HA (RRID: AB_1549585) Cell Signaling Technology 3724 Rabbit monoclonal anti-FLAG (RRID: AB_2572291) Cell Signaling Technology 14793 Mouse monoclonal anti-FLAG (RRID: AB_262044) Sigma Aldrich F1804 Rabbit anti-mTOR (RRID: AB_2105622) Cell Signaling Technology 2983 Rabbit anti-TNPO1 (RRID: AB_3662993) Cell Signaling Technology 31452 Rabbit anti-KPNA2 (RRID: AB_2798467) Cell Signaling Technology 14372 Rabbit anti-IRF4 (RRID: AB_10547141) Cell Signaling Technology 4299 anti-rabbit IgG, HRP-linked (RRID: AB_2099233) Cell Signaling Technology 7074 anti-mouse Alexa 488-conjugated (RRID: AB_2534069) Thermo Fisher Scientific A-11001 anti-FLAG (RRID: AB_2637089) magnetic beads Sigma M8823 anti-HA (RRID: AB_2749815) magnetic beads Thermo Fisher Scientific 88836 M2 FLAG agarose resin Millipore A2220 NeutrAvidin Agarose Thermo Fischer Scientific 29200 Ni-NTA agarose Qiagen 30210 IRDye 800CW Streptavidin LI-COR 926-32230 Bacterial and viral strains Rosetta (DE3) cells Millipore 71397-3 Chemicals, peptides, and recombinant proteins MG132 Selleckchem S2619 PMA Thermo Fisher Scientific J63916.MA Hoechst 33342 Thermo Fisher Scientific H3570 IPTG Sigma I5502-10G Imidazole Sigma 68268-500ML-F Dithiothreitol Thermo Fisher Scientific R0862 Benzonase Millipore 71206-3 HisTEV Thermo Fisher Scientific 12-575-015 Coomassie Brilliant Blue R-250 staining solution Bio Rad 1610436 Tris-Glycine SDS sample buffer Thermo Fisher Scientific LC2676 Immunofluorescence blocking buffer LI-COR 927-70001 ProLong Glass antifade mountant Thermo Fisher Scientific P36982 Protease inhibitor tablets Sigma 11873580001 HALT protease/phosphatase inhibitor Thermo Fisher Scientific 78441 Pierce ECL western blotting substrate Thermo Fisher Scientific 32106 3xFLAG peptide APExBIO A6001 Sequencing Grade Trypsin Promega V5113 Critical commercial assays Cell Titer Glow Promega G9242 PolyJet Transfection Reagent SignaGen SL100688 Q5 Site Directed Mutagenesis kit New England Biolabs E0554S (Continued on next page) e1 Molecular Cell 85, 1–13.e1–e11, July 3, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Gibson Assembly Master Mix New England Biolabs E2611S RNeasy Plus Mini Kit Qiagen 74134 iScript cDNA Synthesis Kit Bio Rad 1708891 Platinum SYBR Green Thermo Fisher Scientific 11733038 S-Trap Micro Proteomics Sample Prep Columns Protifi, LLC C02-micro-10 Deposited data Midnolin-proteasome M1A cryo-EM model and map This paper PDB: 9BUI; EMDB ID: EMD-44910 Midnolin-proteasome M1B cryo-EM model and map This paper PDB: 9BV3; EMDB ID: EMD-44930 Midnolin-proteasome M2A cryo-EM model and map This paper PDB: 9BV1; EMDB ID: EMD-44927 Midnolin-proteasome M2A (focused) cryo-EM map This paper EMDB ID: EMDB ID: EMD-44928 Midnolin-proteasome M2B cryo-EM model and map This paper PDB: 9BV2; EMDB ID: EMD-44929 EGR1-Catch domain crystal structure This paper PDB: 8VQP Raw images and mass spectrometry files This paper Mendeley Data: https://doi.org/10.17632/ xn3xtj2xb7.1 Experimental models: Cell lines HEK-293T ATCC CRL-3216 AMO-1 DSMZ ACC 538 JJN3 DSMZ ACC 541 KMS27 JCRB JCRB1188 KMS26 JCRB JCRB1187 ANBL6 Sigma SCC429

    Immunofluorescence:

    Article Title: Structural basis for the midnolin-proteasome pathway and its role in suppressing myeloma.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Actin (RRID: AB_2223172) Cell Signaling Technology 4970 Rabbit anti-PSMD2 (RRID: AB_2798903) Cell Signaling Technology 25430 Rabbit anti-PSMA2 (RRID: AB_2171400) Cell Signaling Technology 2455 Rabbit anti-PSMB5 (RRID: AB_2798061) Cell Signaling Technology 12919 Rabbit anti-HA (RRID: AB_1549585) Cell Signaling Technology 3724 Rabbit monoclonal anti-FLAG (RRID: AB_2572291) Cell Signaling Technology 14793 Mouse monoclonal anti-FLAG (RRID: AB_262044) Sigma Aldrich F1804 Rabbit anti-mTOR (RRID: AB_2105622) Cell Signaling Technology 2983 Rabbit anti-TNPO1 (RRID: AB_3662993) Cell Signaling Technology 31452 Rabbit anti-KPNA2 (RRID: AB_2798467) Cell Signaling Technology 14372 Rabbit anti-IRF4 (RRID: AB_10547141) Cell Signaling Technology 4299 anti-rabbit IgG, HRP-linked (RRID: AB_2099233) Cell Signaling Technology 7074 anti-mouse Alexa 488-conjugated (RRID: AB_2534069) Thermo Fisher Scientific A-11001 anti-FLAG (RRID: AB_2637089) magnetic beads Sigma M8823 anti-HA (RRID: AB_2749815) magnetic beads Thermo Fisher Scientific 88836 M2 FLAG agarose resin Millipore A2220 NeutrAvidin Agarose Thermo Fischer Scientific 29200 Ni-NTA agarose Qiagen 30210 IRDye 800CW Streptavidin LI-COR 926-32230 Bacterial and viral strains Rosetta (DE3) cells Millipore 71397-3 Chemicals, peptides, and recombinant proteins MG132 Selleckchem S2619 PMA Thermo Fisher Scientific J63916.MA Hoechst 33342 Thermo Fisher Scientific H3570 IPTG Sigma I5502-10G Imidazole Sigma 68268-500ML-F Dithiothreitol Thermo Fisher Scientific R0862 Benzonase Millipore 71206-3 HisTEV Thermo Fisher Scientific 12-575-015 Coomassie Brilliant Blue R-250 staining solution Bio Rad 1610436 Tris-Glycine SDS sample buffer Thermo Fisher Scientific LC2676 Immunofluorescence blocking buffer LI-COR 927-70001 ProLong Glass antifade mountant Thermo Fisher Scientific P36982 Protease inhibitor tablets Sigma 11873580001 HALT protease/phosphatase inhibitor Thermo Fisher Scientific 78441 Pierce ECL western blotting substrate Thermo Fisher Scientific 32106 3xFLAG peptide APExBIO A6001 Sequencing Grade Trypsin Promega V5113 Critical commercial assays Cell Titer Glow Promega G9242 PolyJet Transfection Reagent SignaGen SL100688 Q5 Site Directed Mutagenesis kit New England Biolabs E0554S (Continued on next page) e1 Molecular Cell 85, 1–13.e1–e11, July 3, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Gibson Assembly Master Mix New England Biolabs E2611S RNeasy Plus Mini Kit Qiagen 74134 iScript cDNA Synthesis Kit Bio Rad 1708891 Platinum SYBR Green Thermo Fisher Scientific 11733038 S-Trap Micro Proteomics Sample Prep Columns Protifi, LLC C02-micro-10 Deposited data Midnolin-proteasome M1A cryo-EM model and map This paper PDB: 9BUI; EMDB ID: EMD-44910 Midnolin-proteasome M1B cryo-EM model and map This paper PDB: 9BV3; EMDB ID: EMD-44930 Midnolin-proteasome M2A cryo-EM model and map This paper PDB: 9BV1; EMDB ID: EMD-44927 Midnolin-proteasome M2A (focused) cryo-EM map This paper EMDB ID: EMDB ID: EMD-44928 Midnolin-proteasome M2B cryo-EM model and map This paper PDB: 9BV2; EMDB ID: EMD-44929 EGR1-Catch domain crystal structure This paper PDB: 8VQP Raw images and mass spectrometry files This paper Mendeley Data: https://doi.org/10.17632/ xn3xtj2xb7.1 Experimental models: Cell lines HEK-293T ATCC CRL-3216 AMO-1 DSMZ ACC 538 JJN3 DSMZ ACC 541 KMS27 JCRB JCRB1188 KMS26 JCRB JCRB1187 ANBL6 Sigma SCC429

    Blocking Assay:

    Article Title: Structural basis for the midnolin-proteasome pathway and its role in suppressing myeloma.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Actin (RRID: AB_2223172) Cell Signaling Technology 4970 Rabbit anti-PSMD2 (RRID: AB_2798903) Cell Signaling Technology 25430 Rabbit anti-PSMA2 (RRID: AB_2171400) Cell Signaling Technology 2455 Rabbit anti-PSMB5 (RRID: AB_2798061) Cell Signaling Technology 12919 Rabbit anti-HA (RRID: AB_1549585) Cell Signaling Technology 3724 Rabbit monoclonal anti-FLAG (RRID: AB_2572291) Cell Signaling Technology 14793 Mouse monoclonal anti-FLAG (RRID: AB_262044) Sigma Aldrich F1804 Rabbit anti-mTOR (RRID: AB_2105622) Cell Signaling Technology 2983 Rabbit anti-TNPO1 (RRID: AB_3662993) Cell Signaling Technology 31452 Rabbit anti-KPNA2 (RRID: AB_2798467) Cell Signaling Technology 14372 Rabbit anti-IRF4 (RRID: AB_10547141) Cell Signaling Technology 4299 anti-rabbit IgG, HRP-linked (RRID: AB_2099233) Cell Signaling Technology 7074 anti-mouse Alexa 488-conjugated (RRID: AB_2534069) Thermo Fisher Scientific A-11001 anti-FLAG (RRID: AB_2637089) magnetic beads Sigma M8823 anti-HA (RRID: AB_2749815) magnetic beads Thermo Fisher Scientific 88836 M2 FLAG agarose resin Millipore A2220 NeutrAvidin Agarose Thermo Fischer Scientific 29200 Ni-NTA agarose Qiagen 30210 IRDye 800CW Streptavidin LI-COR 926-32230 Bacterial and viral strains Rosetta (DE3) cells Millipore 71397-3 Chemicals, peptides, and recombinant proteins MG132 Selleckchem S2619 PMA Thermo Fisher Scientific J63916.MA Hoechst 33342 Thermo Fisher Scientific H3570 IPTG Sigma I5502-10G Imidazole Sigma 68268-500ML-F Dithiothreitol Thermo Fisher Scientific R0862 Benzonase Millipore 71206-3 HisTEV Thermo Fisher Scientific 12-575-015 Coomassie Brilliant Blue R-250 staining solution Bio Rad 1610436 Tris-Glycine SDS sample buffer Thermo Fisher Scientific LC2676 Immunofluorescence blocking buffer LI-COR 927-70001 ProLong Glass antifade mountant Thermo Fisher Scientific P36982 Protease inhibitor tablets Sigma 11873580001 HALT protease/phosphatase inhibitor Thermo Fisher Scientific 78441 Pierce ECL western blotting substrate Thermo Fisher Scientific 32106 3xFLAG peptide APExBIO A6001 Sequencing Grade Trypsin Promega V5113 Critical commercial assays Cell Titer Glow Promega G9242 PolyJet Transfection Reagent SignaGen SL100688 Q5 Site Directed Mutagenesis kit New England Biolabs E0554S (Continued on next page) e1 Molecular Cell 85, 1–13.e1–e11, July 3, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Gibson Assembly Master Mix New England Biolabs E2611S RNeasy Plus Mini Kit Qiagen 74134 iScript cDNA Synthesis Kit Bio Rad 1708891 Platinum SYBR Green Thermo Fisher Scientific 11733038 S-Trap Micro Proteomics Sample Prep Columns Protifi, LLC C02-micro-10 Deposited data Midnolin-proteasome M1A cryo-EM model and map This paper PDB: 9BUI; EMDB ID: EMD-44910 Midnolin-proteasome M1B cryo-EM model and map This paper PDB: 9BV3; EMDB ID: EMD-44930 Midnolin-proteasome M2A cryo-EM model and map This paper PDB: 9BV1; EMDB ID: EMD-44927 Midnolin-proteasome M2A (focused) cryo-EM map This paper EMDB ID: EMDB ID: EMD-44928 Midnolin-proteasome M2B cryo-EM model and map This paper PDB: 9BV2; EMDB ID: EMD-44929 EGR1-Catch domain crystal structure This paper PDB: 8VQP Raw images and mass spectrometry files This paper Mendeley Data: https://doi.org/10.17632/ xn3xtj2xb7.1 Experimental models: Cell lines HEK-293T ATCC CRL-3216 AMO-1 DSMZ ACC 538 JJN3 DSMZ ACC 541 KMS27 JCRB JCRB1188 KMS26 JCRB JCRB1187 ANBL6 Sigma SCC429

    Protease Inhibitor:

    Article Title: Structural basis for the midnolin-proteasome pathway and its role in suppressing myeloma.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Actin (RRID: AB_2223172) Cell Signaling Technology 4970 Rabbit anti-PSMD2 (RRID: AB_2798903) Cell Signaling Technology 25430 Rabbit anti-PSMA2 (RRID: AB_2171400) Cell Signaling Technology 2455 Rabbit anti-PSMB5 (RRID: AB_2798061) Cell Signaling Technology 12919 Rabbit anti-HA (RRID: AB_1549585) Cell Signaling Technology 3724 Rabbit monoclonal anti-FLAG (RRID: AB_2572291) Cell Signaling Technology 14793 Mouse monoclonal anti-FLAG (RRID: AB_262044) Sigma Aldrich F1804 Rabbit anti-mTOR (RRID: AB_2105622) Cell Signaling Technology 2983 Rabbit anti-TNPO1 (RRID: AB_3662993) Cell Signaling Technology 31452 Rabbit anti-KPNA2 (RRID: AB_2798467) Cell Signaling Technology 14372 Rabbit anti-IRF4 (RRID: AB_10547141) Cell Signaling Technology 4299 anti-rabbit IgG, HRP-linked (RRID: AB_2099233) Cell Signaling Technology 7074 anti-mouse Alexa 488-conjugated (RRID: AB_2534069) Thermo Fisher Scientific A-11001 anti-FLAG (RRID: AB_2637089) magnetic beads Sigma M8823 anti-HA (RRID: AB_2749815) magnetic beads Thermo Fisher Scientific 88836 M2 FLAG agarose resin Millipore A2220 NeutrAvidin Agarose Thermo Fischer Scientific 29200 Ni-NTA agarose Qiagen 30210 IRDye 800CW Streptavidin LI-COR 926-32230 Bacterial and viral strains Rosetta (DE3) cells Millipore 71397-3 Chemicals, peptides, and recombinant proteins MG132 Selleckchem S2619 PMA Thermo Fisher Scientific J63916.MA Hoechst 33342 Thermo Fisher Scientific H3570 IPTG Sigma I5502-10G Imidazole Sigma 68268-500ML-F Dithiothreitol Thermo Fisher Scientific R0862 Benzonase Millipore 71206-3 HisTEV Thermo Fisher Scientific 12-575-015 Coomassie Brilliant Blue R-250 staining solution Bio Rad 1610436 Tris-Glycine SDS sample buffer Thermo Fisher Scientific LC2676 Immunofluorescence blocking buffer LI-COR 927-70001 ProLong Glass antifade mountant Thermo Fisher Scientific P36982 Protease inhibitor tablets Sigma 11873580001 HALT protease/phosphatase inhibitor Thermo Fisher Scientific 78441 Pierce ECL western blotting substrate Thermo Fisher Scientific 32106 3xFLAG peptide APExBIO A6001 Sequencing Grade Trypsin Promega V5113 Critical commercial assays Cell Titer Glow Promega G9242 PolyJet Transfection Reagent SignaGen SL100688 Q5 Site Directed Mutagenesis kit New England Biolabs E0554S (Continued on next page) e1 Molecular Cell 85, 1–13.e1–e11, July 3, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Gibson Assembly Master Mix New England Biolabs E2611S RNeasy Plus Mini Kit Qiagen 74134 iScript cDNA Synthesis Kit Bio Rad 1708891 Platinum SYBR Green Thermo Fisher Scientific 11733038 S-Trap Micro Proteomics Sample Prep Columns Protifi, LLC C02-micro-10 Deposited data Midnolin-proteasome M1A cryo-EM model and map This paper PDB: 9BUI; EMDB ID: EMD-44910 Midnolin-proteasome M1B cryo-EM model and map This paper PDB: 9BV3; EMDB ID: EMD-44930 Midnolin-proteasome M2A cryo-EM model and map This paper PDB: 9BV1; EMDB ID: EMD-44927 Midnolin-proteasome M2A (focused) cryo-EM map This paper EMDB ID: EMDB ID: EMD-44928 Midnolin-proteasome M2B cryo-EM model and map This paper PDB: 9BV2; EMDB ID: EMD-44929 EGR1-Catch domain crystal structure This paper PDB: 8VQP Raw images and mass spectrometry files This paper Mendeley Data: https://doi.org/10.17632/ xn3xtj2xb7.1 Experimental models: Cell lines HEK-293T ATCC CRL-3216 AMO-1 DSMZ ACC 538 JJN3 DSMZ ACC 541 KMS27 JCRB JCRB1188 KMS26 JCRB JCRB1187 ANBL6 Sigma SCC429

    Western Blot:

    Article Title: Structural basis for the midnolin-proteasome pathway and its role in suppressing myeloma.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Actin (RRID: AB_2223172) Cell Signaling Technology 4970 Rabbit anti-PSMD2 (RRID: AB_2798903) Cell Signaling Technology 25430 Rabbit anti-PSMA2 (RRID: AB_2171400) Cell Signaling Technology 2455 Rabbit anti-PSMB5 (RRID: AB_2798061) Cell Signaling Technology 12919 Rabbit anti-HA (RRID: AB_1549585) Cell Signaling Technology 3724 Rabbit monoclonal anti-FLAG (RRID: AB_2572291) Cell Signaling Technology 14793 Mouse monoclonal anti-FLAG (RRID: AB_262044) Sigma Aldrich F1804 Rabbit anti-mTOR (RRID: AB_2105622) Cell Signaling Technology 2983 Rabbit anti-TNPO1 (RRID: AB_3662993) Cell Signaling Technology 31452 Rabbit anti-KPNA2 (RRID: AB_2798467) Cell Signaling Technology 14372 Rabbit anti-IRF4 (RRID: AB_10547141) Cell Signaling Technology 4299 anti-rabbit IgG, HRP-linked (RRID: AB_2099233) Cell Signaling Technology 7074 anti-mouse Alexa 488-conjugated (RRID: AB_2534069) Thermo Fisher Scientific A-11001 anti-FLAG (RRID: AB_2637089) magnetic beads Sigma M8823 anti-HA (RRID: AB_2749815) magnetic beads Thermo Fisher Scientific 88836 M2 FLAG agarose resin Millipore A2220 NeutrAvidin Agarose Thermo Fischer Scientific 29200 Ni-NTA agarose Qiagen 30210 IRDye 800CW Streptavidin LI-COR 926-32230 Bacterial and viral strains Rosetta (DE3) cells Millipore 71397-3 Chemicals, peptides, and recombinant proteins MG132 Selleckchem S2619 PMA Thermo Fisher Scientific J63916.MA Hoechst 33342 Thermo Fisher Scientific H3570 IPTG Sigma I5502-10G Imidazole Sigma 68268-500ML-F Dithiothreitol Thermo Fisher Scientific R0862 Benzonase Millipore 71206-3 HisTEV Thermo Fisher Scientific 12-575-015 Coomassie Brilliant Blue R-250 staining solution Bio Rad 1610436 Tris-Glycine SDS sample buffer Thermo Fisher Scientific LC2676 Immunofluorescence blocking buffer LI-COR 927-70001 ProLong Glass antifade mountant Thermo Fisher Scientific P36982 Protease inhibitor tablets Sigma 11873580001 HALT protease/phosphatase inhibitor Thermo Fisher Scientific 78441 Pierce ECL western blotting substrate Thermo Fisher Scientific 32106 3xFLAG peptide APExBIO A6001 Sequencing Grade Trypsin Promega V5113 Critical commercial assays Cell Titer Glow Promega G9242 PolyJet Transfection Reagent SignaGen SL100688 Q5 Site Directed Mutagenesis kit New England Biolabs E0554S (Continued on next page) e1 Molecular Cell 85, 1–13.e1–e11, July 3, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Gibson Assembly Master Mix New England Biolabs E2611S RNeasy Plus Mini Kit Qiagen 74134 iScript cDNA Synthesis Kit Bio Rad 1708891 Platinum SYBR Green Thermo Fisher Scientific 11733038 S-Trap Micro Proteomics Sample Prep Columns Protifi, LLC C02-micro-10 Deposited data Midnolin-proteasome M1A cryo-EM model and map This paper PDB: 9BUI; EMDB ID: EMD-44910 Midnolin-proteasome M1B cryo-EM model and map This paper PDB: 9BV3; EMDB ID: EMD-44930 Midnolin-proteasome M2A cryo-EM model and map This paper PDB: 9BV1; EMDB ID: EMD-44927 Midnolin-proteasome M2A (focused) cryo-EM map This paper EMDB ID: EMDB ID: EMD-44928 Midnolin-proteasome M2B cryo-EM model and map This paper PDB: 9BV2; EMDB ID: EMD-44929 EGR1-Catch domain crystal structure This paper PDB: 8VQP Raw images and mass spectrometry files This paper Mendeley Data: https://doi.org/10.17632/ xn3xtj2xb7.1 Experimental models: Cell lines HEK-293T ATCC CRL-3216 AMO-1 DSMZ ACC 538 JJN3 DSMZ ACC 541 KMS27 JCRB JCRB1188 KMS26 JCRB JCRB1187 ANBL6 Sigma SCC429

    Sequencing:

    Article Title: Structural basis for the midnolin-proteasome pathway and its role in suppressing myeloma.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Actin (RRID: AB_2223172) Cell Signaling Technology 4970 Rabbit anti-PSMD2 (RRID: AB_2798903) Cell Signaling Technology 25430 Rabbit anti-PSMA2 (RRID: AB_2171400) Cell Signaling Technology 2455 Rabbit anti-PSMB5 (RRID: AB_2798061) Cell Signaling Technology 12919 Rabbit anti-HA (RRID: AB_1549585) Cell Signaling Technology 3724 Rabbit monoclonal anti-FLAG (RRID: AB_2572291) Cell Signaling Technology 14793 Mouse monoclonal anti-FLAG (RRID: AB_262044) Sigma Aldrich F1804 Rabbit anti-mTOR (RRID: AB_2105622) Cell Signaling Technology 2983 Rabbit anti-TNPO1 (RRID: AB_3662993) Cell Signaling Technology 31452 Rabbit anti-KPNA2 (RRID: AB_2798467) Cell Signaling Technology 14372 Rabbit anti-IRF4 (RRID: AB_10547141) Cell Signaling Technology 4299 anti-rabbit IgG, HRP-linked (RRID: AB_2099233) Cell Signaling Technology 7074 anti-mouse Alexa 488-conjugated (RRID: AB_2534069) Thermo Fisher Scientific A-11001 anti-FLAG (RRID: AB_2637089) magnetic beads Sigma M8823 anti-HA (RRID: AB_2749815) magnetic beads Thermo Fisher Scientific 88836 M2 FLAG agarose resin Millipore A2220 NeutrAvidin Agarose Thermo Fischer Scientific 29200 Ni-NTA agarose Qiagen 30210 IRDye 800CW Streptavidin LI-COR 926-32230 Bacterial and viral strains Rosetta (DE3) cells Millipore 71397-3 Chemicals, peptides, and recombinant proteins MG132 Selleckchem S2619 PMA Thermo Fisher Scientific J63916.MA Hoechst 33342 Thermo Fisher Scientific H3570 IPTG Sigma I5502-10G Imidazole Sigma 68268-500ML-F Dithiothreitol Thermo Fisher Scientific R0862 Benzonase Millipore 71206-3 HisTEV Thermo Fisher Scientific 12-575-015 Coomassie Brilliant Blue R-250 staining solution Bio Rad 1610436 Tris-Glycine SDS sample buffer Thermo Fisher Scientific LC2676 Immunofluorescence blocking buffer LI-COR 927-70001 ProLong Glass antifade mountant Thermo Fisher Scientific P36982 Protease inhibitor tablets Sigma 11873580001 HALT protease/phosphatase inhibitor Thermo Fisher Scientific 78441 Pierce ECL western blotting substrate Thermo Fisher Scientific 32106 3xFLAG peptide APExBIO A6001 Sequencing Grade Trypsin Promega V5113 Critical commercial assays Cell Titer Glow Promega G9242 PolyJet Transfection Reagent SignaGen SL100688 Q5 Site Directed Mutagenesis kit New England Biolabs E0554S (Continued on next page) e1 Molecular Cell 85, 1–13.e1–e11, July 3, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Gibson Assembly Master Mix New England Biolabs E2611S RNeasy Plus Mini Kit Qiagen 74134 iScript cDNA Synthesis Kit Bio Rad 1708891 Platinum SYBR Green Thermo Fisher Scientific 11733038 S-Trap Micro Proteomics Sample Prep Columns Protifi, LLC C02-micro-10 Deposited data Midnolin-proteasome M1A cryo-EM model and map This paper PDB: 9BUI; EMDB ID: EMD-44910 Midnolin-proteasome M1B cryo-EM model and map This paper PDB: 9BV3; EMDB ID: EMD-44930 Midnolin-proteasome M2A cryo-EM model and map This paper PDB: 9BV1; EMDB ID: EMD-44927 Midnolin-proteasome M2A (focused) cryo-EM map This paper EMDB ID: EMDB ID: EMD-44928 Midnolin-proteasome M2B cryo-EM model and map This paper PDB: 9BV2; EMDB ID: EMD-44929 EGR1-Catch domain crystal structure This paper PDB: 8VQP Raw images and mass spectrometry files This paper Mendeley Data: https://doi.org/10.17632/ xn3xtj2xb7.1 Experimental models: Cell lines HEK-293T ATCC CRL-3216 AMO-1 DSMZ ACC 538 JJN3 DSMZ ACC 541 KMS27 JCRB JCRB1188 KMS26 JCRB JCRB1187 ANBL6 Sigma SCC429

    Transfection:

    Article Title: Structural basis for the midnolin-proteasome pathway and its role in suppressing myeloma.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Actin (RRID: AB_2223172) Cell Signaling Technology 4970 Rabbit anti-PSMD2 (RRID: AB_2798903) Cell Signaling Technology 25430 Rabbit anti-PSMA2 (RRID: AB_2171400) Cell Signaling Technology 2455 Rabbit anti-PSMB5 (RRID: AB_2798061) Cell Signaling Technology 12919 Rabbit anti-HA (RRID: AB_1549585) Cell Signaling Technology 3724 Rabbit monoclonal anti-FLAG (RRID: AB_2572291) Cell Signaling Technology 14793 Mouse monoclonal anti-FLAG (RRID: AB_262044) Sigma Aldrich F1804 Rabbit anti-mTOR (RRID: AB_2105622) Cell Signaling Technology 2983 Rabbit anti-TNPO1 (RRID: AB_3662993) Cell Signaling Technology 31452 Rabbit anti-KPNA2 (RRID: AB_2798467) Cell Signaling Technology 14372 Rabbit anti-IRF4 (RRID: AB_10547141) Cell Signaling Technology 4299 anti-rabbit IgG, HRP-linked (RRID: AB_2099233) Cell Signaling Technology 7074 anti-mouse Alexa 488-conjugated (RRID: AB_2534069) Thermo Fisher Scientific A-11001 anti-FLAG (RRID: AB_2637089) magnetic beads Sigma M8823 anti-HA (RRID: AB_2749815) magnetic beads Thermo Fisher Scientific 88836 M2 FLAG agarose resin Millipore A2220 NeutrAvidin Agarose Thermo Fischer Scientific 29200 Ni-NTA agarose Qiagen 30210 IRDye 800CW Streptavidin LI-COR 926-32230 Bacterial and viral strains Rosetta (DE3) cells Millipore 71397-3 Chemicals, peptides, and recombinant proteins MG132 Selleckchem S2619 PMA Thermo Fisher Scientific J63916.MA Hoechst 33342 Thermo Fisher Scientific H3570 IPTG Sigma I5502-10G Imidazole Sigma 68268-500ML-F Dithiothreitol Thermo Fisher Scientific R0862 Benzonase Millipore 71206-3 HisTEV Thermo Fisher Scientific 12-575-015 Coomassie Brilliant Blue R-250 staining solution Bio Rad 1610436 Tris-Glycine SDS sample buffer Thermo Fisher Scientific LC2676 Immunofluorescence blocking buffer LI-COR 927-70001 ProLong Glass antifade mountant Thermo Fisher Scientific P36982 Protease inhibitor tablets Sigma 11873580001 HALT protease/phosphatase inhibitor Thermo Fisher Scientific 78441 Pierce ECL western blotting substrate Thermo Fisher Scientific 32106 3xFLAG peptide APExBIO A6001 Sequencing Grade Trypsin Promega V5113 Critical commercial assays Cell Titer Glow Promega G9242 PolyJet Transfection Reagent SignaGen SL100688 Q5 Site Directed Mutagenesis kit New England Biolabs E0554S (Continued on next page) e1 Molecular Cell 85, 1–13.e1–e11, July 3, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Gibson Assembly Master Mix New England Biolabs E2611S RNeasy Plus Mini Kit Qiagen 74134 iScript cDNA Synthesis Kit Bio Rad 1708891 Platinum SYBR Green Thermo Fisher Scientific 11733038 S-Trap Micro Proteomics Sample Prep Columns Protifi, LLC C02-micro-10 Deposited data Midnolin-proteasome M1A cryo-EM model and map This paper PDB: 9BUI; EMDB ID: EMD-44910 Midnolin-proteasome M1B cryo-EM model and map This paper PDB: 9BV3; EMDB ID: EMD-44930 Midnolin-proteasome M2A cryo-EM model and map This paper PDB: 9BV1; EMDB ID: EMD-44927 Midnolin-proteasome M2A (focused) cryo-EM map This paper EMDB ID: EMDB ID: EMD-44928 Midnolin-proteasome M2B cryo-EM model and map This paper PDB: 9BV2; EMDB ID: EMD-44929 EGR1-Catch domain crystal structure This paper PDB: 8VQP Raw images and mass spectrometry files This paper Mendeley Data: https://doi.org/10.17632/ xn3xtj2xb7.1 Experimental models: Cell lines HEK-293T ATCC CRL-3216 AMO-1 DSMZ ACC 538 JJN3 DSMZ ACC 541 KMS27 JCRB JCRB1188 KMS26 JCRB JCRB1187 ANBL6 Sigma SCC429

    Mutagenesis:

    Article Title: Structural basis for the midnolin-proteasome pathway and its role in suppressing myeloma.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-Actin (RRID: AB_2223172) Cell Signaling Technology 4970 Rabbit anti-PSMD2 (RRID: AB_2798903) Cell Signaling Technology 25430 Rabbit anti-PSMA2 (RRID: AB_2171400) Cell Signaling Technology 2455 Rabbit anti-PSMB5 (RRID: AB_2798061) Cell Signaling Technology 12919 Rabbit anti-HA (RRID: AB_1549585) Cell Signaling Technology 3724 Rabbit monoclonal anti-FLAG (RRID: AB_2572291) Cell Signaling Technology 14793 Mouse monoclonal anti-FLAG (RRID: AB_262044) Sigma Aldrich F1804 Rabbit anti-mTOR (RRID: AB_2105622) Cell Signaling Technology 2983 Rabbit anti-TNPO1 (RRID: AB_3662993) Cell Signaling Technology 31452 Rabbit anti-KPNA2 (RRID: AB_2798467) Cell Signaling Technology 14372 Rabbit anti-IRF4 (RRID: AB_10547141) Cell Signaling Technology 4299 anti-rabbit IgG, HRP-linked (RRID: AB_2099233) Cell Signaling Technology 7074 anti-mouse Alexa 488-conjugated (RRID: AB_2534069) Thermo Fisher Scientific A-11001 anti-FLAG (RRID: AB_2637089) magnetic beads Sigma M8823 anti-HA (RRID: AB_2749815) magnetic beads Thermo Fisher Scientific 88836 M2 FLAG agarose resin Millipore A2220 NeutrAvidin Agarose Thermo Fischer Scientific 29200 Ni-NTA agarose Qiagen 30210 IRDye 800CW Streptavidin LI-COR 926-32230 Bacterial and viral strains Rosetta (DE3) cells Millipore 71397-3 Chemicals, peptides, and recombinant proteins MG132 Selleckchem S2619 PMA Thermo Fisher Scientific J63916.MA Hoechst 33342 Thermo Fisher Scientific H3570 IPTG Sigma I5502-10G Imidazole Sigma 68268-500ML-F Dithiothreitol Thermo Fisher Scientific R0862 Benzonase Millipore 71206-3 HisTEV Thermo Fisher Scientific 12-575-015 Coomassie Brilliant Blue R-250 staining solution Bio Rad 1610436 Tris-Glycine SDS sample buffer Thermo Fisher Scientific LC2676 Immunofluorescence blocking buffer LI-COR 927-70001 ProLong Glass antifade mountant Thermo Fisher Scientific P36982 Protease inhibitor tablets Sigma 11873580001 HALT protease/phosphatase inhibitor Thermo Fisher Scientific 78441 Pierce ECL western blotting substrate Thermo Fisher Scientific 32106 3xFLAG peptide APExBIO A6001 Sequencing Grade Trypsin Promega V5113 Critical commercial assays Cell Titer Glow Promega G9242 PolyJet Transfection Reagent SignaGen SL100688 Q5 Site Directed Mutagenesis kit New England Biolabs E0554S (Continued on next page) e1 Molecular Cell 85, 1–13.e1–e11, July 3, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Gibson Assembly Master Mix New England Biolabs E2611S RNeasy Plus Mini Kit Qiagen 74134 iScript cDNA Synthesis Kit Bio Rad 1708891 Platinum SYBR Green Thermo Fisher Scientific 11733038 S-Trap Micro Proteomics Sample Prep Columns Protifi, LLC C02-micro-10 Deposited data Midnolin-proteasome M1A cryo-EM model and map This paper PDB: 9BUI; EMDB ID: EMD-44910 Midnolin-proteasome M1B cryo-EM model and map This paper PDB: 9BV3; EMDB ID: EMD-44930 Midnolin-proteasome M2A cryo-EM model and map This paper PDB: 9BV1; EMDB ID: EMD-44927 Midnolin-proteasome M2A (focused) cryo-EM map This paper EMDB ID: EMDB ID: EMD-44928 Midnolin-proteasome M2B cryo-EM model and map This paper PDB: 9BV2; EMDB ID: EMD-44929 EGR1-Catch domain crystal structure This paper PDB: 8VQP Raw images and mass spectrometry files This paper Mendeley Data: https://doi.org/10.17632/ xn3xtj2xb7.1 Experimental models: Cell lines HEK-293T ATCC CRL-3216 AMO-1 DSMZ ACC 538 JJN3 DSMZ ACC 541 KMS27 JCRB JCRB1188 KMS26 JCRB JCRB1187 ANBL6 Sigma SCC429

    Incubation:

    Article Title: In vitro activity of cysteamine against SARS-CoV-2 variants
    Article Snippet: .. Cytotoxicity was measured by adding 100 μl of Cell titer glow (Promega) reagent and incubated for 15 min at room temperature. ..

    Article Title: G3BP1 inhibits Cul3 SPOP to amplify AR signaling and promote prostate cancer.
    Article Snippet: .. On day six, cell viability was assessed by adding 100 μL per well of cell titer glow (Promega) followed by a 10 min incubation at 37 °C and measured by the amount of luminescence using a 96-well plate luminometer (GlowMax; Promega). ..



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